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KMID : 1011420230280010027
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Journal of Korean Ophthalmic Optics Society
2023 Volume.28 No. 1 p.27 ~ p.36
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Immunocytochemical Localization of Cholinergic Amacrine Cells in the Mongolian Gerbil Retina: Colocalization with Calcium-Binding Protein Calretinin
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Kim Kang-Ho
Son Jae-Rim
Kwon Oh-Ju
Jeon Chang-Jin
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Abstract
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Purpose : To study the distribution pattern of cholinergic amacrine cells in the diurnal Mongolian gerbil retina.
Methods : Immunocytochemistry and confocal microscopy were used to investigate the localization of choline acetyltransferase-immunoreactive (ChAT-IR) cells in the gerbil retina. The cell density of ChAT-IR cells was quantitatively analyzed.
Results : ChAT-IR cells were present in the inner nuclear layer and the ganglion cell layer; the processes of these cells form two distinct narrow bands in the inner plexiform layer, as previously reported in various animals. The average density of ChAT-IR cells in the ganglion cell layer was 1,242±111 cells/mm2 and 1,487±257 cells/mm2 in the inner nuclear layer, demonstrating the number of ChAT-IR cells in the inner nuclear layer was higher than that in the ganglion cell layer. The cholinergic amacrine cells in the gerbil retina contained the calcium-binding protein calretinin but not calbindin-D28K or parvalbumin.
Conclusions : The distributional pattern shows that cholinergic amacrine cells in the gerbil retina are similar to those in other animals. Calcium-binding proteins expression in cholinergic amacrine cells are species-specific. The cholinergic amacrine cells play key roles in the motion detection mechanisms in the retina. Therefore, this study on the gerbil retina may provide better information for further physiological studies involving a diurnal animal model.
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KEYWORD
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Mongolian gerbil, Retina, Cholinergic amacrine cells, Calcium-binding proteins, Immunocytochemistry
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